Sontakke Shital, N. B. Ghiware, S. K. Sarje, Phytochemical and pharmacological evaluation of vigna radiata stem bark extracts for antiamnetic activity, ASIO Journal of Experimental Pharmacology & Clinical Research (ASIO-JEPCR), 2020, 6(1): 54-68.
ARTICLE TYPE: RESEARCH
Affiliation: Department of Pharmacology, Nanded Pharmacy College, Nanded, Maharashtra, India.
DOI Link :: http://doi-ds.org/doilink/08.2020-49964865/
The present study reports physicochemical characterization, antioxidant and Antiamnetic activity of extracts from vigna radiata stem bark collected from local region of Nanded, Maharashtra, India. Different physical parameters like ash values, extractive value, Loss on drying, solubility etc were evaluated for powdered drug. The extracts were obtained from Soxhlet method by using ethyl acetate and methanol as solvents for extraction and subjected for preliminary physicochemical evaluation and antioxidant studies. Total phenolic and flavonoids content were also analyzed. The presence of primary and secondary metabolites such as carbohydrate, proteins, alkaloids, phenolic compounds, saponins was confirmed through preliminary phyto-chemical analysis. DPPH free radical scavenging assays showed strong antioxidant activities with increase in concentration of ethyl acetate and methanol stem bark extracts. Maximum percentage inhibition i.e. 80.97% was shown by ethyl acetate extract at concentration of 150 μg/ml and was compared with Ascorbic acid as reference standard. The In-Vivo Antiamnetic activity of vigna radiata stem bark was evaluated by radial arm maze model in rats using Piracetam as a standard. Scopalamine (1mg/kg) used as inducing agent. Both the extracts at 200mg/kg concentrations showed significant to highly significant number of entries & time spent in P zone (from P < 0.05 to P < 0.001). The result suggests that vigna ridiata stem bark extracts possess Antiamnetic avtivity and this might be due to flavonids. Phenolic compound, steroid and proteins present in extract.
Keywords: Vigna radiata, ethyl acetate and Methanolic extract, Phytochemical screening, Antioxidant effect, Antiamnetic activity.
. Abhinav Kanwal Jogender Mehla, Madhusudana Kunchal, Vegi Ganga Modi Naidul, Yogendra Kumar Gupta, Ramakrishna Sistla, Anti-Amnesic Activity of Vitex Negundo in Scopolamine Induced Amnesia in Rats Pharmacology & Pharmacy, 2010, 1: 1-8.
. Kumar Ganesan; A critical review on phytochemical profile and health promotingeffects of mung bean (Vigna radiata), Food Science and Human Wellness 2018, 7: 11–33.
. Khandelwal K. R. Practical Pharmacognosy NiraliPrakashan, 21th edition, 2010, 25:1-25.9.
. Biju J, Sulajman CT, Satheesh G, Reddy VR. Total Phenolics and Flavonoids in Selected Medicinal Plants from Kerala. Int J of Phar and Pharm. Sci., 2014, 6(1): 406-408.
. US, Muhammad MA, Azwani MS, Nasyriq A. Determination of Total Phenolics, Flavonoids Content and Free Radical Scavenging Activities of Common Herbs and Spices. J of Pharmacognosy and Phytochemistry, 2014, 3(3): 104-108.
. Dildar A, Muhammad MK, Ramsha S. Comparative Analysis of Phenolics, Flavonoids, Antioxidant and Antibacterial Potential of Methanolic, Hexanic and Aqueous Extracts from Adiantum Caudatum Leaves. Antioxidants, 2015, 4: 394-409.
. Reena Randhir, Stimulation of phenolics, antioxidant and antimicrobial activities in dark germinated mung bean sprouts in response to peptide and phytochemical elicitors process biochemistry, 2004, 39: 637-646.
. H. Gerhard Vogel, Drug discovery & evaluation, Pharmacological assays, II edition, 2002, 936-944.
. Samira Rohanama Zhara Robiei, Zahra Alibabbaei et al. antiamnetic activity on C. aurantium flowers extract against Scopolamine-induced memory impairments in rats, Neural Sci., 2015, 6: 553-560.
. Lee SJ, Lee JH, Lee HH, Lee S, Kim SH, Chun T, Imm JY. Effect of mung bean ethanol extract on pro-inflammtory cytokines in LPS stimulated macrophages. Food Sci Biotechnol., 2011, 20(2): 519–524.
. Dongyan Tang, Yinmao Dong, Hankun Ren , Li Li and Congfen He. A review of phytochemistry, metabolite changes, and medicinal uses of the common food mung bean and its sprouts Vigna radiate, Tang et al. Chemistry Central Journal 2014, 3: 8-4.