Kharate Pooja, N. B. Ghiware, S. K. Sarje, Phytochemical and pharmacological evaluation of terminalia catappa linn. leaves extracts for antianxiety activity, ASIO Journal of Experimental Pharmacology & Clinical Research (ASIO-JEPCR), 2020, 6(1): 79-91.
ARTICLE TYPE: RESEARCH
Affiliation: Department of Pharmacology, Nanded Pharmacy College, Nanded, Maharashtra, India.
DOI Link :: http://doi-ds.org/doilink/08.2020-53693327/
The present study investigate physicochemical characterization, antioxidant and Antianxiety activity of extracts from Terminalia catappa leaves collected from local region of Nanded, Maharashtra, India. Different physical parameters like ash values, extractive value, Loss on drying, solubility etc were evaluated for powdered drug. The extracts were obtained from Soxhlet method by using Acetone and methanol as solvents for extraction and subjected for preliminary physicochemical evaluation and antioxidant studies. Total phenolic and flavonoids content were also analyzed. The presence of phytoconstituents such as carbohydrate, proteins, alkaloids, phenolic compounds, saponins was confirmed through preliminary phyto-chemical analysis. DPPH free radical scavenging assays showed strong antioxidant activities with increase in concentration of acetone and methanol leaves extracts. Maximum percentage inhibition i.e. 80.97% was shown by methanolic extract at concentration of 150 μg/ml and was compared with Ascorbic acid as reference standard. The In-Vivo Antianxiety activity of Terminalia catappa leaves was evaluated by Elevated plus maze and Light and Dark model in rats using Diazepam as a standard. Both the extracts at 200mg/kg concentration showed significant to highly significant number of entries & time spent in P zone (from P < 0.05 to P < 0.001). The study reveals that Terminalia catappa leaves extracts possess Antianxiety activity and this might be due to flavonoids. Phenolic compound, steroid and proteins present in extract.
Keywords: Terminalia catappa, Acetone and Methanolic extract, Phytochemical screening, Antioxidant effect, Antianxiety activity.
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