Albert Science International Organization

ARTICLE DESCRIPTION: 

Kharate Pooja, N. B. Ghiware, S. K. Sarje, Phytochemical and pharmacological evaluation of terminalia catappa linn. leaves extracts for antianxiety activity, ASIO Journal of Experimental Pharmacology & Clinical Research (ASIO-JEPCR), 2020, 6(1): 79-91.

ARTICLE TYPE: RESEARCH

Affiliation: Department of Pharmacology, Nanded Pharmacy College, Nanded, Maharashtra, India. 

Doi: 10.2016-37245516/;

DOI Link :: http://doi-ds.org/doilink/08.2020-53693327/

ABSTRACT:

The present study investigate physicochemical characterization, antioxidant and Antianxiety  activity of extracts from Terminalia catappa leaves collected from local region of Nanded, Maharashtra, India. Different physical parameters like ash values, extractive value, Loss on drying, solubility etc were evaluated for powdered drug. The extracts were obtained from Soxhlet method by using Acetone and methanol as solvents for extraction and subjected for preliminary physicochemical evaluation and antioxidant studies. Total phenolic and flavonoids content were also analyzed. The presence of phytoconstituents such as carbohydrate, proteins, alkaloids, phenolic compounds, saponins was confirmed through preliminary phyto-chemical analysis. DPPH free radical scavenging assays showed strong antioxidant activities with increase in concentration of acetone and methanol leaves extracts. Maximum percentage inhibition i.e. 80.97% was shown by methanolic extract at concentration of 150 μg/ml and was compared with Ascorbic acid as reference standard. The In-Vivo Antianxiety activity of Terminalia catappa leaves was evaluated by Elevated plus maze and Light and Dark model in rats using Diazepam as a standard. Both the extracts at 200mg/kg concentration showed significant to highly significant number of entries & time spent in P zone (from P < 0.05 to P < 0.001). The study reveals that Terminalia catappa leaves extracts possess Antianxiety activity and this might be due to flavonoids. Phenolic compound, steroid and proteins present in extract.

Keywords: Terminalia catappa, Acetone and Methanolic extract, Phytochemical screening, Antioxidant effect, Antianxiety activity.  

[1]. Thibaut F. The role of sex and gender in neuropsychiatric disorders. Dialogues Clin Neurosci. 2016:18(4), 351-352.

[2]. Thibaut F. Gender does matter in clinical research. Eur Arch Psychiatry Clin Neurosci. 2017,267(4):283-284.

[3]. D.S. Mohale, A. P. Dewani, A. V. Chandewar, C. D. Khadse, A.S. Tripathi, S.S. Agrawal, Brief Review on Medicinal Potential Of Terminalia Catappa Journal of Herbal Medicine and Toxicology, 2009: 3 (1), 7-11.

[4]. Biju J, Sulajman CT, Satheesh G, Reddy VR. Total Phenolics and Flavonoids in Selected Medicinal Plants from Kerala. Int J of Phar and Pharm Sci 2014: 6(1): 406-408.

[5].US, Muhammad MA, Azwani MS, Nasyriq A. Determination of Total Phenolics, Flavonoids Content and Free Radical Scavenging Activities of Common Herbs and Spices. J of Pharmacognosy and Phytochemistry, 2014; 3(3): 104-108.

[6].Dildar A, Muhammad MK, Ramsha S. Comparative Analysis of Phenolics, Flavonoids, Antioxidant and Antibacterial Potential of Methanolic, Hexanic and Aqueous Extracts from Adiantum Caudatum Leaves. Antioxidants, 2015; 4: 394-409.

[7]. Shaik GP, Shaik KS, Shaik YA, Imaduddin, Roshan S, Saffon AK. Evaluation of Antiepileptic and Antioxidant Activity of Methanolic Extract of Terminalia Tomentosa (Roxb) Wight and Arn in Rats. World J of Pharm Res., 2015; 4(2): 766-776.

[8]. H. Gerhard Vogel, Drug discovery & evaluation, Pharmacological assays, II edition, 2002; 936-944.

[9]. Shahina Naz, Samia Ahmad, 2Sheikh Ajaz Rasool, Rahmanullah Siddiqi and Syed Asad Sayeed, :Res J Microbiol, 2007: 2, 180-184.

[10]. Gilman, E.F., Watson, D. G, Terminalia catappa. Tropical- Almond Fact Sheet ST 1994: 3, 626-629.

[11]. Deshmukh Shachi, S. K. Sarje, N. B. Ghiware, Phytochemical and pharmacological evaluation ofterminalia catappa linn. leaves extracts for antiamnetic activity, ASIO Journal of Experimental Pharmacology & Clinical Research (ASIO-JEPCR), 2020, 6(1): 69-78.