Shinde Punam, N. B Ghiware, S. K. Sarje, Shaikh Arshad, Phytochemical and pharmacological evaluation of ruellia tuberosa stem bark extracts for antidepressant activity, ASIO Journal of Experimental Pharmacology & Clinical Research (ASIO-JEPCR), 2020, 6(1): 109-119.
ARTICLE TYPE: RESEARCH
Affiliation: Department of Pharmacology, Nanded Pharmacy College, Nanded, Maharashtra, India.
DOI Link :: http://doi-ds.org/doilink/08.2020-47838591/
The present study reports physicochemical characterization, antidepressant activity of extracts from Ruellia tuberosa Stem Bark collected from local region of Nanded, Maharashtra, India. Different physical parameters like ash values, extractive value, Loss on drying, solubility etc were evaluated for powdered drug. The extracts were obtained from Soxhlet method by using water and methanol as solvents for extraction and subjected for preliminary physicochemical evaluation and antioxidant studies. Total phenolic and flavonoids content were also analyzed. The presence of primary and secondary metabolites such as carbohydrate, proteins, alkaloids, phenolic compounds, saponins was confirmed through preliminary phyto-chemical analysis. DPPH free radical scavenging assays showed strong antioxidant activities with increase in concentration of Ethyl acetate and methanol leaf extracts. Maximum percentage inhibition i.e. 80.97% was shown by methanolic extract at concentration of 150 μg/ml and was compared with Ascorbic acid as reference standard. The In-Vivo antidepressant activity of Ruellia tuberosa Stem Bark was evaluated by Despair Swim Test model in rats using Imipramine as a standard. Both the extracts at 200mg/kg Concentration showed significant to highly significant number of entries & time spent in P zone (from P < 0.05 to P <0.001). The result suggest that Ruellia tuberosa Stem Bark extracts possess antidepressant activity and this might be due to flavonids. Phenolic compound, steroid and proteins present in extract.
Keywords: Ruellia Tuberosa Stem Bark, Ethyl acetate and Methanolic extract, Phytochemical screening, Antioxidant effect, antidepressant activity.
. Iyer K. and Khan Z.A, Depression – A Review, Research Journal of Recent Sciences, 2012: 1(4), 79-87.
. Supawana Khachitpongpanit, Supawatchara Singhatong, Thanapat Sastraruji and Churdsak Jaikang, Phytochemical study of Ruellia tuberosa chloroform extract: antioxidant and anticholinesterase activities, Der Pharmacia Lettre, 2016: 8 (6), 238-244.
. A. safitri, Sutrisno, A. Roosdiana, C.A. Evindasari, Hypoglycaemic activity of hydroethanolic root extracts of Ruellia tuberosa L. in diabetic rats,Journal of physics 2018:3,(1),1-6.
. Khandelwal K. R. Practical Pharmacognosy NiraliPrakashan, 21th edition, 2010: 25.1-25.9
. Biju J, Sulajman CT, Satheesh G, Reddy VR. Total Phenolics and Flavonoids in Selected Medicinal Plants from Kerala. Int J of Phar and Pharm Sci 2014: 6(1), 406-408.
. US, Muhammad MA, Azwani MS, Nasyriq A. Determination of Total Phenolics, Flavonoids Content and Free Radical Scavenging Activities of Common Herbs and Spices. J of Pharmacognosy and Phytochemistry, 2014: 3(3), 104-108.
. Dildar A, Muhammad MK, Ramsha S. Comparative Analysis of Phenolics, Flavonoids, Antioxidant and Antibacterial Potential of Methanolic, Hexanic and Aqueous Extracts from Adiantum Caudatum Leaves. Antioxidants, 2015: 4, 394-409.
. Shaik GP, Shaik KS, Shaik YA, Imaduddin, Roshan S, Saffon AK. Evaluation of Antiepileptic and Antioxidant Activity of Methanolic Extract of Terminalia Tomentosa (Roxb) Wight and Arn in Rats. World J of Pharm Res., 2015: 4(2) 766-776.
. H. Gerhard Vogel, Drug discovery & evaluation, Pharmacological assays, II edition, 2002: 936-944.
.The wealth of India Vol. IX, (Council of Industrial and Scientific Research, New Delhi). 1972: 2(1), 90-96.
. Daya L Chothani, Patel MB, Mishra SH, Vaghasiya Hc, Daya L Chothani, Patel MB, Mishra SH, Vaghasiya H, Phcog J 2010:2 (12), 506–512.
. Thakur Adhika Govindsinh, M.H. Ghante, N. B. Ghiware, S.K. Sarje, Shagufta Farooqui,, Pharmacological evaluation of ruellia tuberosa stem bark on antianxiety activity, ASIO Journal of Experimental Pharmacology & Clinical Research (ASIO-JEPCR), 2020, 6(1): 102-108.