ARTICLE DESCRIPTION: 

Yong-Chol Hong, Ju-WonKim, Un-SonPaek, Jong-Hwa Jin, Effect of resveratrol in endometritis; experimental study, ASIO Journal of Experimental Pharmacology & Clinical Research (ASIO-JEPCR), 2025, 8(1): 01-07.

Yong-Chol Hong, Ju-WonKim, Un-SonPaek, Jong-Hwa Jin

Department of Obstetrics and Gynecology, Pyongyang University of Medical Sciences, Ryonhwa Dong, Central District, Pyongyang, Democratic People’s Republic of Korea.

DOI Link ::

Abstract:

The aim of the present study is to evaluate the treatment potential of resveratrol in an experimentally induced endometritis model in rats.

Background: Endometritis mostly progresses sub clinically and cause infertility through the disruption of the hormonal balance. It has been shown in many studies that resveratrol has anti-inflammatory and antioxidant properties. However, the possible beneficial effects of resveratrol inendometritis have not been determined yet.

Object: Endometritis was induced in adult female Wistar rats (200~220g).

Methodology: To induce endometritis, 16mg/kg/s.c. progesterone was given for 5 days, and then Escherichia coli (50μl, 1×10⁵cfu/rat) was injected in the right cornu uteri following laparotomy.20 hours after bacterial inoculation, the treatment protocol (Resveratrol, 50mg/kg i.m.) was applied for 14 days.

Result: From 20 h after E. coli inoculation, there were significant differences between the control and study groups in various parameters, and at 14th day of treatment, the treatment parameters were not significantly differences between the study group and control group.

Conclusion: According to the results of the current study, resveratrol was found to be effective in the treatment of endometritis with its antioxidant and anti-inflammatory functions.

Key words: Endometritis, Resveratrol.

[1] Sheldon IM, Dobson H (2004) Postpartum uterine health in cattle. Anim Reprod Sci 82-83:295–306

[2] Adnane M, Kaidi R, Hanzen C, England GCW (2017) Risk factors of clinical and subclinical endometritis in cattle: a review. Turk J Vet Anim Sci 41:1–11

[3] Salah N, Yimer N (2017) Cytological endometritis and its agreement with ultrasound examination in postpartum beef cows. Vet World 10(6): 605–609

[4] Prunner I, Pothmann H, Wagener K, Giuliodori M, Huber J, EhlingSchulz M, Drillich M (2014) Dynamics of bacteriologic and cytologic changes in the uterus of postpartum dairy cows. Theriogenology 82:1316–1322

[5] Xu D, Xiong H, Xiao Z, He J, Liao Q, Xue L, Wang N, Yang Q (2015) Uterine cytokine profile in a rat model of endometritis. Am J Reprod Immunol 73(3):214–220

[6] Jang M, Cai L, Udeani GO, Slowing KV, Thomas CF, Beecher CW, Fong HH, Farnsworth NR, Kinghorn AD, Mehta RG, Moon RC, Pezzuto JM (1997) Cancer chemoprotective activity of resveratrol, a natural product derived from grapes. Science 275:218–220.

[7] Amaya SC, Savaris RF, Filipovic CJ, Wise JD, Hestermann E, Young SL, Lessey BA (2014) Resveratrol and endometrium: a closer look at an active ingredient of red wine using in vivo and in vitro models. Reprod Sci 21(11):1362–1369.

[8] Poulsen MM, Fjeldborg K, Ornstrup MJ, Kjær TN, Nøhr MK, Pedersen SB (2015) Resveratrol and inflammation: challenges in translating pre-clinical findings to improved patient outcomes. Biochim Biophys Acta 1852(6):1124–1136.

[9] Salehi B, Mishra AP, Nigam M, Sener B, Kilic M, Sharifi-Rad M, Fokou PVT, Martins N, Sharifi-Rad J (2018) Resveratrol: a double-edged sword in health benefits. Biomedicines 6(3):E91.

[10] Smoliga JM, Baur JA, Hausenblas HA (2011) Resveratrol and health–a comprehensive review of human clinical trials. Mol Nutr Food Res 55(8):1129–1141.

[11] Liu M, Yin Y, Ye X, Zeng M, Zhao Q, Keefe DL, Liu L (2013) Resveratrol protects against age-associated infertility in mice. Hum Reprod 28(3):707–717.

[12] Parkinson T (2001) Infertility in the cow. In: Noakes DE, Parkinson TJ, England GCW (eds) Arthur’s veterinary reproduction and obstetrics, 8th edn. Saunders Company, Philadelphia, pp 383–471

[13] Palmer C (2014) Postpartum uterine infection. In: Hopper RM (ed) Bovine reproduction. 1st ed. Chapter 50. John Wiley & Sons, Inc. pp 440–448

[14] McFarland J (1907) Nephelometer: an instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines. J Am Med Assoc 14:1176–1178.

[15] Forbes BA, Sahm DF, Weissfeld AS (2002) Laboratory methods for detection of antibacterial resistance. In: Bailey & Scott’s diagnostic microbiology. Eleventh edn. St. Louis: Mosby Inc; 2002.; pp. 230–231

[16] Hughes VM, Stevenson K, Sharp JM (2001) Improved preparation of high molecular weight DNA for pulsed-field gel electrophoresis from mycobacteria. J Microbiol Methods 44(3):209–215.

[17] Khare S, Ficht TA, Santos RL, Romano J, Ficht AR, Zhang S, Grant IR, Libal M, Hunter D, Adams LG (2004) Rapid and sensitive detection of Mycobacterium avium subsp. paratuberculosis in bovine milk and feces by a combination of immunomagnetic bead separationconventional PCR and real-time PCR. J Clin Microbiol 42(3):1075–1081.

[18] Mikamo H, Kawazoe K, Sato Y, Izumi K, Tamaya T (1998) Therapeutic effects of a new quinolone, DU-6859a, on polymicrobial infections in a newly designed model of rat uterine endometritis. J Antimicrob Chemother 41(1):131–133

[19] Winterbourn CC, Hawkins RE, Brian M, Carrell RW (1975) The estimation of red cell superoxide dismutase activity. J Lab Clin Med 85:337–341

[20] Aebi H (1974) Catalase. In: Bergmeyer HU (ed) Method in enzymology, 2, Academic press, New York, pp 673–678

[21] Yoshioka T, Kawada K, Shimada T, et al. Lipid peroxidation in maternal and cord blood and protective mechanisms against activated oxygen toxicity in the blood. Am J Obster Gynecol 1979;135:372–6.

[22] Westergren A. The technique of the red cell sedimentation reaction. Am Rev Tuberc 1926; 14: 94–101

[23] Kitaya K, Yasuo T, Tada Y, Hayashi T, Taguchi S, Funabiki M, Nakamura Y (2013) Current understanding of chronic endometritis. Diagn Histopathol 19(7):231–237.

[24] Liang Y, Shen T, Ming Q, Han G, Zhang Y, Liang J, Zhu D (2018) Alpinetin ameliorates inflammatory response in LPS-induced endometritis in mice. Int Immunopharmacol 62:309–312.

[25] Wu H, Dai A, Chen X, Yang X, Li X, Huang C, Jiang K, Deng G (2018) Leonurine ameliorates the inflammatory responses in lipopolysaccharide-induced endometritis. Int Immunopharmacol 61:156–161.

[26] Phyu HE, Irwin JC, Vella RK, Fenning AS (2016) Resveratrol shows neuronal and vascular-protective effects in older, obese, streptozotocin-induced diabetic rats. Br J Nutr 115(11):1911–1918

[27] Pessoa AS, Aguiar GPS, Oliveira JV, Bortoluzzi AJ, Paulino A, Lanzaa M (2019) Precipitation of resveratrol-isoniazid and resveratrolnicotinamide cocrystals by gas antisolvent. J Supercrit Fluids 145:93–102.

[28] Tabibiazar M, Mohammadifar MA, Roufegarinejad L, Ghorbani M, Hashemi M, Hamishehkar H (2019) Improvement in dispersibility, stability and antioxidant activity of resveratrol using a colloidal nanodispersion of BSA-resveratrol. Food Biosci 27:46–53.

[29] Lim CG, Fowler ZL, Hueller T, Schaffer S, Koffas MAG (2011) Highyield resveratrol production in engineered Escherichia coli. Appl Environ Microbiol 77(10):3451–3460.

[30] Hwang D, Lim YH (2015) Resveratrol antibacterial activity against Escherichia coli is mediated by Z-ring formation inhibition via suppression of FtsZ expression. Sci Rep 5:10029.

[31] Ma DSL, Tan LT, Chan KG, Yap WH, Pusparajah P, Chuah LH, Ming LC, Khan TM, Lee LH, Goh BH (2018) Resveratrol-potential antibacterial agent against foodborne pathogens. Front Pharmacol 9:102.

[32] Singh D, Mendonsa R, Koli M, Subramanian M, Nayak SK (2019) Antibacterial activity of resveratrol structural analogues: a mechanistic evaluation of the structure-activity relationship. Toxicol Appl Pharmacol 367:23–32.

[33] Nishikawa Y, Baba T, Imori T (1984) Effect of the estrous cycle on uterine infection induced by Escherichia coli. Infect Immun 43(2): 678–683

[34] Sheldon IM, Dobson H (2004) Postpartum uterine health in cattle. Anim Reprod Sci 82-83:295–306.

[35] Smith M, Hagerty KA, Skipper B, Bocklage T (2010) Chronic endometritis: a combined histopathologic and clinical review of cases from 2002 to 2007. Int J Gynecol Pathol 29(1):44–50.

[36] Kitaya K, Yasuo T, Tada Y, Hayashi T, Taguchi S, Funabiki M, Nakamura Y (2013) Current understanding of chronic endometritis. Diagn Histopathol 19(7):231–237.

[37] Lugrin J, Rosenblatt-Velin N, Parapanov R, Liaudet L (2014) The role of oxidative stress during inflammatory processes. Biol Chem 395(2): 203–230.

[38] Hussain T, Tan B, Yin Y, Blachier F, Tossou MC, Rahu N (2016) Oxidative stress and inflammation: what polyphenols can do for us? Oxidative Med Cell Longev 2016:7432797–7432799.

[39] Heidarpour M, Mohri M, Fallah-Rad AH, Shahreza FD, Mohammadi M (2012) Oxidative stress and trace elements before and after treatment in dairy cows with clinical and subclinical endometritis. Revue Méd Vét 163(12):628–633

[40] Galvão KN, Santos JEP (2014) Recent advances in the immunology and uterine microbiology of healthy cows and cows that develop uterine disease. Turk J Vet Anim Sci 38:577–588.

[41] Gülçin ? (2010) Antioxidant properties of resveratrol: a structure–activity insight. Innov Food Sci Emerg Technol 11(1):210–218.

[42] Orallo F (2006) Comparative studies of the antioxidant effects of cis- and trans-resveratrol. Curr Med Chem 13(1):87–98.

[43] Xia N, Daiber A, Förstermann U, Li H (2017) Antioxidant effects of resveratrol in the cardiovascular system. Br J Pharmacol 174(12): 1633–1646.

[44] Oh WY, Shahidi F (2018) Antioxidant activity of resveratrol ester derivatives in food and biological model systems. Food Chem 261:267–273.