Albert Science International Organization

ARTICLE DESCRIPTION: 

Manisha Masih, Abhilasha Mittal, B.C. Nandy, Development and Validation of HPLC Method for Simultaneous Estimation of Amlodipine Besylate and Lisinopril DihydrateiIn Solid Dosage Form, ASIO Journal of Analytical Chemistry (ASIO-JAC), 2015, 1(1): 33-37.

ARTICLE TYPE: RESEARCH

dids no.: 03.2016-28554435,

dids link:  http://dids.info/didslink/03.2016-64176365/

ABSTRACT:

A rapid, sensitive and specific HPLC method involving UV detection was developed and validated for determination and quantification of Amlodipine besylate and Lisinopril dihydrate in tablet dosage form. The determination was carried out on a Phenomenex C18 (250 x 4.6 mm, 5 μm) column using filtered and degassed mixture of methanol: 1N HCl (1:1) as mobile phase at a flow rate of 1 ml/min and effluent was monitored at 230 nm. The retention time for Amlodipine besylate was 4.20 min and for Lisinopril dihydrate 2.76 min. Amlodipine besylate and Lisinopril dihydrate showed a linear response in the concentration range of 10-50μg/ml. The correlation co-efficient ('r' value) for Amlodipine besylate and Lisinopril dihydrate was 0.9997 and 0.9989, respectively. The method was validated in terms of linearity, precision, accuracy, specificity, robustness and solution stability. The proposed method can be useful in the quality control of bulk manufacturing and pharmaceutical dosage forms.

Keywords: Amlodipine besylate, Lisinopril dihydrate, Method development, Validation, HPLC.

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