Sounik Banerjee,Tamal Maity, Amlan Bishal, Rupajit Bhattacharjee, Satya Prasanna Das, Moumita Maji, Effect of Ascorbic Acid on Estimation of Oxidative Stress by Measuring the Level of Glutathione in the Serum of Rabbit’s Blood, ASIO Journal of Experimental Pharmacology & Clinical Research (ASIO-JEPCR), 2016, 1(1): 23-26.
ARTICLE TYPE: RESEARCH
dids no.: 03.2016-35559215,
dids Link: http://dids.info/didslink/03.2016-36744879/
Ascorbic acid is known as hexuronic acid. Some epidemiological data mentioned its usefulness in reducing cold with increasing consumption of foods rich in vitamin, so people occasionally consume an overdose of it. Ascorbic acid is a well-known antioxidant & it has good antioxidant prospective. The term vitamin C is generally employed to explain all these compounds, although the representative of which is ascorbic acid. Its antioxidant capability is connected with reduced incidence of cancer. In the present study, effects of Ascorbic acid on blood Malonaldehyde (MDA) level was evaluated in an attempt to find its anti-peroxidative potential. Plan of the experiment being in an in-vivo system using New Zealand white rabbit as an animal of choice. Rabbit was chosen for this work as it is a mammal & having similarity with human beings. It was also selected due to its easy availability & ease of experiment. Estimation of Oxidative Stress was performed by measuring the level of Glutathione in the serum of rabbit’s blood before and after the administration of Ascorbic Acid. Estimation was made by using a Standard Curve given below. Though the results clearly supports the antioxidant capacity of ascorbic acid, but in some experiments it is found that ascorbic acid also has pro-oxidant effect. A final conclusion cannot be drawn considering this little volume of work. We can reach in a final conclusion only after repeated experiments using more animal models.
Keywords: Vitamin C, GSH, Antioxidants, blood MDA level
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