Albert Science International Organization

ARTICLE DESCRIPTION: 

P. S. Bairy, A Comparative Study of HPLC and HPTLC: Principles, Instrumentations and Applications, ASIO Journal of Analytical Chemistry (ASIO-JAC), 2015, 1(1): 20-28.

ARTICLE TYPE: REVIEW

dids no.: 03.2016-28554435,

dids Link:    http://dids.info/didslink/03.2016-91512171/

ABSTRACT:

The analytical method should be sensitive, specific, fast and accurate to establish the assurance that the equipments used in manufacturing are free of the unwanted impurity, presence of which may alter the safety and efficacy of the drug product. HPLC, UPLC techniques have established their role in pharmaceutical cleaning validation. Among the modern Analytical tools HPTLC is a powerful analytical method equally suitable for qualitative and quantitative analytical tasks. HPTLC is playing an important role in today analytical world, not in competition to HPLC but as a complementary method. One of the most obvious orthogonal features of the two techniques is the primary use of reversed phases in HPLC versus unmodified silica gel in HPTLC, resulting in partition chromatography and adsorption chromatography respectively. High Performance Thin layer Chromatography (HPTLC) technique is a sophisticated and automated form of the thin-layer chromatography (TLC) with better and advanced separation efficiency and detection limits, and is often an excellent alternative to GC and HPLC. Applications of HPTLC include phytochemical and biomedical analysis, herbal drug quantification, active ingredient quantification, fingerprinting of formulations, and check for adulterants in the formulations. HPTLC is also useful in detecting chemicals of forensic concern. 

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