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JOURNALS || ASIO Journal of Experimental Pharmacology & Clinical Research (ASIO-JEPCR) [ISSN: 2455-7080]

Author Names : Sounik Banerjee
Page No. : 23-26  volume 1 issue 1
Article Overview


Sounik Banerjee,Tamal Maity, Amlan Bishal, Rupajit Bhattacharjee, Satya Prasanna Das, Moumita Maji, Effect of Ascorbic Acid on Estimation of Oxidative Stress by Measuring the Level of Glutathione in the Serum of Rabbit’s Blood, ASIO Journal of Experimental Pharmacology & Clinical Research (ASIO-JEPCR), 2016, 1(1): 23-26.


dids no.: 03.2016-35559215,

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Ascorbic acid is known as hexuronic acid. Some epidemiological data mentioned its usefulness in reducing cold with increasing consumption of foods rich in vitamin, so people occasionally consume an overdose of it. Ascorbic acid is a well-known antioxidant & it has good antioxidant prospective. The term vitamin C is generally employed to explain all these compounds, although the representative of which is ascorbic acid. Its antioxidant capability is connected with reduced incidence of cancer. In the present study, effects of Ascorbic acid on blood Malonaldehyde (MDA) level was evaluated in an attempt to find its anti-peroxidative potential. Plan of the experiment being in an in-vivo system using New Zealand white rabbit as an animal of choice. Rabbit was chosen for this work as it is a mammal & having similarity with human beings. It was also selected due to its easy availability & ease of experiment. Estimation of Oxidative Stress was performed by measuring the level of Glutathione in the serum of rabbit’s blood before and after the administration of Ascorbic Acid. Estimation was made by using a Standard Curve given below. Though the results clearly supports the antioxidant capacity of ascorbic acid, but in some experiments it is found that ascorbic acid also has pro-oxidant effect. A final conclusion cannot be drawn considering this little volume of work. We can reach in a final conclusion only after repeated experiments using more animal models.

Keywords: Vitamin C, GSH, Antioxidants, blood MDA level

  1. Okumura H, Nishimura E, Kariya S. Angiotensin-converting enzyme (ACE) [No relation between angiotensin-converting enzyme (ACE) inhibitor-induced cough and ACE gene polymer-phism, plasma bradykinin, substance P and ACE inhibitor concentration in Japanese patients]. Yakugaku Zasshi, 2001, 121(3): 253-7.  
  2.  Thomas MC. Diuretics, ACE inhibitors and NSAIDs - the triple whammy. Med J Aust, 2000, 172(4):184–185.
  3.  Molinaro G, Cugno M, Perez M. Angiotensin-converting enzyme inhibitor-associated angioedema is characterized by a slower degradation of des-arginine(9)-bradykinin, J Pharmacol Exp Ther., 2002, 303:232-7.
  4. FitzGerald RJ, Murray BA, Walsh DJ. Hypotensive peptides from milk proteins. J Nutr., 2004,134: 980S-8S.
  5.  Aihara K, Kajimoto O, Hirata H, Takahashi R, Nakamura Y. Effect of powdered fermented milk with Lactobacillus helveticus on subjects with high-normal blood pressure or mild hypertension, J Am Coll Nutr., 2005, 24(4):257-65
  6. Cooper WO, Hernandez-Diaz S, Arbogast PG, Dudley JA, Dyer S, Gideon PS, et al. Major congenital malformations after first-trimester exposure to ACE inhibitors, N Engl J Med., 2006, 354(23):2443-51.
  7. Luno J, Praga M, De Vinuesa SG. The reno-protective effect of the dual blockade of the renin angiotensin system (RAS), Curr Pharm Des., 2005, 11(10):1291-300.
  8.  Van de Wal RM, Van Veldhuisen DJ, Van Gilst WH, Voors AA. Addition of an angiotensin receptor blocker to full-dose ACE-inhibition: controversial or common sense? Eur Heart J. 2005, 26(22):2361-7
  9. Lowry OH, Rosebrough NJ, Farr AL, Randal RJ. Protein measurement with Folin phenol reagent, J Biol Chem., 1951, 193: 265-275.
  10. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase, Anal Biochem., 1986, 16: 359-364.
  11. Goldberg DM, Spooner RG. Glutathione reductase. In: Bergmeyer J, Grabl M, eds. Methods of enzymatic analysis, Chemie, Deerfield Beach, 1983, 258-265.
  12. Whanger PD, Butler JA. Effects of various dietary levels of selenium as selenite or selenomethionine on tissue selenium and glutathione peroxidase activity in rats, J Nutr., 1988, 7: 846-852.
  13. Paoletti F, Aldinucci D, Mocali A, Caparrini A. A sensitive spectrophotometric method for the determination of superoxide dismutase activity in tissue extracts, Anal Biochem., 1986, 154: 536-541.
  14. Ross MA. Determination of ascorbic acid and uric acid in plasma by high-perphormance liquid chromatography, J Chromatogr., 1994, 576: 221-233.
  15. Burton RH, Rice Evans AC. Free radicals and regulation of mammalian cell proliferation, Free Radic Res Commun, 1989, 6: 345-358.
  16. Chopra S, Wallace HM. Induction of spermidine/spermine N1-acetyl transferase in humen cancer cells in response-increased production of reactive oxygen species, Biochem Pharmacol., 1998, 55: 1119-1123.