Albert Science International Organization

ABSTRACT

This study evaluated the microbial quality of well water used in Amassoma, Bayelsa state. Triplicate water samples were collected from eight wells in Amassoma. Standard microbiological procedures were used for determining the microbial diversity and density. Results showed that fecal, total coliform, total heterotrophic bacteria and total Staphylococci counts ranged from 2.00 to 13.67 MPN/100ml, 4.03 to 23.33 MPN/100ml, 0.59 to 35.23 x10⁴ cfu/ml and 6.7 to 60.67 x10¹ cfu/ml respectively. Analysis of variance showed that there were significance differences (P<0.05) among the various location for total heterotrophic bacteria and total coliform and no significance difference (P>0.05) among the various locations for fecal coliform and Staphylococci counts. Based on density, the concentration exceeded World Health Organization/Food and Agricultural Organization allowable limit of 1.0 x 10² cfu/ml for potable water. Also it exceeded the Standard Organization of Nigeria maximum permissible level of 10cfu/ml for total coliform. The bacteria isolated includes Pseudomonas, Enterobacter, Bacillus, Micrococcus, Serratia, Streptococcus and Proteus species, Staphylococcus aureus and E. coli. The similarity of the isolated bacteria diversity between the locations based on Sorenson qualitative index ranged from 42.86 – 92.31%. Due to the occurrence of these microbes and the higher population occurring in the water, its suitability for consumption is doubtful. Appropriate treatment, such as chlorination was recommended for the sanitization of the water.

Keywords: Amassoma, Bacteria, water quality, well water.

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