Albert Science International Organization

ARTICLE DESCRIPTION: 

Rania M. A. Elmahe, Nabila. E. Yousif and Elhadi. A. I. Elkhalil, The potentiality of Saccharomyces Cerevisiae to produce single cell oil (SCO) from wheat straw and molasses, ASIO Journal of Microbiology, Food Science & Biotechnological Innovations (ASIO-JMFSBI), 2020, 5(1): 16-22.

^1&2 Department of Food Science and Technology, Faculty of Agriculture, University of Khartoum, 13314, Shambat, Sudan

³Department of Botany & Agric. Biotechnology, Faculty of Agriculture, University of Khartoum, 13314, Shambat, Sudan

Doi: 10.2016-53692176; DOI Link :: http://doi-ds.org/doilink/11.2020-75125565/

ABSTRACT:

The study aimed to explore the use of isolated Sacchromyces cerevisiae for single cell oil production using wheat straw and molasses as a cheaper source of nitrogen and sugars. A total of 10 samples of Sacchromyces cerevisiae were used, isolated from rotten fruit and fruit juice, the effectiveness of them to produce the single cell oil was studied, when the yeast was grown on wheat straw, detoxified liquid hydrolysate (DLH) and nondetoxified liquid hydrolysate (NDLH) and molasses. The productivity of single cell oil produced by Sacchromyces cerevisiae strains were different according to the sources of isolation. Sacchromyces cerevisiae isolated from rotten apple gave high oil productivity 84.05%, of lipids on sugar cane molasses medium and 50.7 % on wheat straw medium.

Keywords: Oleaginous yeast, single cell oil, DLH: detoxified liquid hydrolysate

1. Ratledge, C. and Wynn J.P. The biochemistry and molecular biology of lipid accumulation in oleaginous microorganisms. Adv. Appl. Microbiol, 2002, 51:1–51.
2. Ratledge, C. Fatty acid biosynthesis in microorganisms being used for Single Cell Oil production. Biochimie, 2004, 15: 86- 807.
3. Sijtsma, L. and Swaaf, M. Biotechnological production and applications of the -3 polyunsaturated fatty acid docosahexaenoic acid. Appl. Microbiol Biotechnol, 2004, 64: 53- 146.
4. Beopoulos, A. and Nicaud, J. M. An overview of lipid metablism in yeasts and its impact on biotechnological processes.Appl. Microbiol Biotechnol, 2009, 90: 1193-1206.
5. Alvarez, H.M. and Steinbuchel, A. Triacylglycerols in prokaryotic microorganisms. Appl. Microbiol Biotechnol, 2002, 60: 367–376.
6. Tehlivets, O.; Scheuringer, K. and Kohlwein, SD. Fatty acids synthesis and elongation in yeast. BBA-Mol Cell Biol L, 2007, 70: 177-255.
7. Meng, X.; Yang, J.; Xu, X.; Zhang, L.; Nie, Q. and Xian, M. Biodiesel production from oleaginous microorganisms. Renew. Energy, 2009, 34: 1-5.
8. Li, YQ; Horsman, M.; Wang, B.; Wu, N. and Lan, CQ. Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans. Appl Microbiol Biotechnol, 2008, 81: 29-36.
9. MAF. Ministry of Agriculture and Forestry. Agricultural Policies Section. Annual report 2008, SOBA, Sudan.
10. Thiru, M.; Sankh, S. and Rangaswamy, V. Process for biodiesel production from Cryptococcus curvatus. Biores. Technol, 2011, 102 (22):10436-10440.
11. Ratledge, C. and Cohen, Z. Microbial and algal oils: Do they have a future for biodiesel or as commodity oils? Lipid Tech, 2008, 20: 155-160.
12. Morgunov, IG.; Solodovnikova, NY.; Sharyshev, AA.; and Kamzolova, SV. FinogenovaTV.Regulation of NAD (+)-Dependent isocitrate dehydrogenase in the citrate producing yeast Yarrowiali polytica. Biochemistry-Moscow, 2004, 69: 39-84.
13. Zhang, Y.; Adams, IP. and Ratledge, C. Malic enzyme: the controlling activity for lipid production Overexpression of malic enzyme in Mucorcircinelloidesleads to a 2.5- fold increase in lipid accumulation. Microbiology-SGM, 2007, 153: 20-25.
14. Burton, M.; Rose, TM; Faergeman, NJ. And Knudsen, J. Evolution of the Acyl-CoA binding protein (ACBP). Biochem J, 2005, 392: 299-307.
15. Wu, S.T.; Yu, S.T. and Lin, L. P. Effect of culture conditions on docosahexaenoic acid production by Schizochytrium sp S31.Process Biochem, 2005,  40: 31-83.
16. Peng, X. and Chen, H. Rapid estimation of single cell oil content of solid-state fermented mass using near-infrared spectroscopy. Bioresour Technol, 2008, 99: 72-8869.
17. Rajoka, M.I. The enzymatic hydrolysis and fermentation of pretreated wheat straw and baggase to ethanol. Afr. Tech. Dev. Forum, 2005, 2(2): 29-35.
18. Reith, J.H.; Den Uil, H.; Van Veen, W.T.; De Laat, J.J.; Niessen and De Jong, E. Coproduction of bioethanol, electricity and heat from biomass residues.12th European Conference and Technology Exhibition on Biomass from Energy. Industry and Climate Protection, Amsterdam, Netherlands, 2002.
19. Papanikolaou, S. and Aggelis, G. Lipids of oleaginous yeasts. Part I.  Biochemistry of single cell oil production. Eur J Lipid Sci Technol, 2011, 113: 51–103.
20. Papanikolaou, S.; Chevalot, I.; Komaitis, M.; Aggelis, G. and Marc, I. Kinetic profile of the cellular lipid composition in an oleaginous Yarrowialipolytica capable of producing a cocoa-butter substitute from industrial fats. Anton Leeuw Int. J. G, 2001, 80: 24-215.
21. Talebnia, F.; Karakashev, D. and Angelidaki, I. Production of bioethanol from wheat straw: an overview on pretreatment, hydrolysis and fermentation. Bioresour. Technol, 2010, 101: 4744–4753.
22. Tehmina, K. and Umarah, M. Wheat Straw: A Pragmatic Overview. Department of Biological Sciences, Forman Christian College (A Chartered University), Ferozpur Road, Lahore 54600, Pakistan, 2012.
23. Saha, B.C.; Iten, L.B.; Cotta, M.A. and Wu, Y.V. Dilute acid pretreatment, enzymatic saccharification and fermentation of wheat straw to ethanol. Process Biochem. Oxford.UK, 2005, 40: 3693-3700.
24. Taherzadeh, M.J. and Karimi, K. Pretreatment of lignocelluloses wastes to improve ethanol and biogas production: a review. Int. J. Mol. Sci, 2008, 9: 1621–1651.
25. Chen, Y.; Sharma-Shivappa, R.R.; Keshwani, D.; and Chen, C. Potential of agricultural residues and hay for bioethanol production. Appl. Biochem. Biotechnol, 2009, 142: 276-290.
26. Zhu, L.Y.; Zong, M. H. and Wu, H. Efficient lipid production with Trichosporon fermentans and its use for biodiesel preparation.Bioresour. Technol, 2008, 99: 7881-7885.
27. Chatzifragkou, A.; Fakas, S.; Galiotou-anayotou, M.; Komaitis, M.; Aggelis, G. and Papanikolaou, S. Commercial sugars as substrates for lipid accumulation in Cunninghamella echinulata and Mortierella isabellina fungi. Eur J Lipid Sci Technol, 2010, 57: 112-148
28. Mohagheghi, A.; Ruth, M. and Schell, D. J. Conditioning hemicellulose hydrolysates for fermentation: effects of overliming pH on sugar and ethanol yields.Process Biochem.  Amsterdam. Neth, 2006, 41:1806-1811.
29. Huang, C.; Zong, M. H.; Wu, H. and Liu, Q. P. Microbial oil production from rice straw hydrolysate by Trichosporon fermentans. Bioresour. Technol, 2009, 100:4535– 4538.
30. Husain, A.; Noura, E. and El-Sayed M. Single cell oil production by an oleaginous yeast strain in a low cost cultivation medium. Research Journal of Microbiology, 2010, 4 (8):301-313.
31. Li, M.; Liu, G.L.; Chi, Z. and Chi, Z.M. Single cell oil production from hydrolysate of cassava starch by marine-derived yeast Rhodotorulamucilaginosa.Biom.Bioenergy, 2010, 4: 101-107.
32. Hu, C.; Zhao, X.; Zhao, J.; Wu, S. and Zhao, Z.K. Effects of biomass hydrolysis byproducts on oleaginous yeast Rhodosporidium toruloides. Bioresour. Technol, 2009, 100:4843–4847.
33. Xiaochen, Y.u.; Yubin, Z.; Kathleen, M. and Shulin.C. Oil production by oleaginous yeasts using the hydrolysate from pretreatment of wheat straw with dilute sulfuricacid.Bioresour. Technol, 2011, 102:6134-6140.
34. Thidarat, P.; Supaporn, K. and Ratanaporn, L. Microbial Oil Production by Monoculture and Mixed Cultures of Microalgae and Oleaginous Yeasts using Sugarcane molasses as Substrate. Engineering and Technology International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering, 2012, 6: 1-4.
35. Gajdoš, P.; Nicaud, JM. and Rossignol, T. Single cell oil production on molasses by Yarrowia lipolytica strains overexpressing DGA2 in multicopy. Microbial Biotechnol, 2015, 99: 65-80.