Albert Science International Organization

ARTICLE DESCRIPTION: 

Vishal Galave, Kajal Nadaf, Pravin Metkari, Priyanka Nangare, Amruta Menkudale, Priyanka Surushe, Blotting methods for the investigation of DNA, RNA, and proteins, ASIO Journal of Microbiology, Food Science & Biotechnological Innovations (ASIO-JMFSBI), 2021, 6(1): 01-08.

Department of Pharmaceutics, Navsahyadri Institute of Pharmacy, Naigaon( Nasarapur), Pune

Doi: 10.2016-53692176; 

DOI Link :: http://doi-ds.org/doilink/05.2021-65846166/ASIO-JMFSBI/2021/525

ABSTRACT:

Blotting is a typical procedure which are southern, western, northern and eastern are relevant for various kinds of macromolecules like lipids, RNA, DNA and proteins.. Western blotching (or immunoblotting) is a generally utilized method to distinguish proteins just as posttranslational changes on proteins, utilizing counter acting agent based tests to get clear data about objective proteins from multifaceted examples. Southern blotching is the first smearing strategy, which began the naming framework. It was intended by Edwin Southern. The Southern smear is utilized to recognize the measure of DNA in a blend. The term 'northern smudge' really alludes explicitly to the hair like exchange of RNA from the electrophoresis gel to the smearing layer. The eastern smear, or eastern blotching, is a biochemical method used to look at protein post-translational modifications (PTM) including the development of lipids, phosphates, and glycoconjugates. It is normally used to recognize sugar epitopes.

 Keywords: Southern, Northern, Western Blotting, Probe, Hybridization, Antibody, Membrane.

1. Tani Y, et al. Evaluation of a Novel Automated Machine, the Auto2D, for Two-Dimensional Gel Electrophoresis. J Proteomics Bioinform. 2014;7:108-111 .
2.  Marcel Binnebösel, et al. Gentamicin supplemented polyvinylidenfluoride mesh materials enhance tissue integration due to a transcriptionally reduced MMP-2 protein expression BMC Surgery. 2012;121-122
3. Li A. Encoding Genetic Information and Catalyzing Chemical Reactions: The Multifunctional Roles of Nucleic Acids. Hereditary Genet. 2015;4O-45
4.  Polly P, et al. Evaluation of an adaptive virtual laboratory environment using Western Blotting for diagnosis of disease. BMC Medical Education. 2014;14:222..
5. Aboud O and Griffin W. Silver Staining of Alzheimer's Disease. J NeurolDisord. 2014;2:i103. 
6. Itodo SE, et al. Phytochemical Properties and Staining Ability of Red Onion (Allium cepa) Extract on Histological Sections. J CytolHistol. 2014;5:275
7. Manu Tomar, “Types of Blotting”, Research & Reviews: Journal of Pharmaceutics and Nanotechnology, Vol . 4 , 30-09-2016 , Page No. 147
8. https://en.wikipedia.org/wiki/File:Blotting_Compass_for_Molecular_Probes.png.
9. https://images.app.goo.gl/USWXejqkqhTZocbJA.]
10. http://thebumblingbiochemist.com/365-days-of-science/blotting-compass-northern-eastern-southern-western-blots-more/. 
11. https://en.wikipedia.org/wiki/Blot_(biology)
12. https://images.app.goo.gl/oTmca1uFrRJxWw7V9
13. https://www.mybiosource.com/learn/westernblotting
14. https://www.bosterbio.com/protocol-and-troubleshooting/western-blot-principle.
15. https://www.creative-proteomics.com/blog/index.php/the-principle-and-procedure-of-western-blot/)[
16. https://www.sciencedirect.com/science/article/pii/S000326971930750X
17. "Test Blots, Slot Blots & Dot Blots - Immunodetection | Bio-Rad". Bio-Rad
18. https://en.wikipedia.org/wiki/Dot_blot)
19. Rupprecht, Kevin R.; Nair, Rad K.; Harwick, Larissa C.; Grote, Jonathan; Beligere, Gangamani S.; Rege, Sushil D.; Chen, Yon-Yih; Lin, Zhen; Fishpaugh, Jeffrey R. (15 December 2010). "Development of a dot-blot assay for screening monoclonal antibodies to low-molecular-mass drugs". Analytical Biochemistry: 160–164. doi:10.1016/j.ab.2010.08.003. PMID 20696124
20. https://www.mybiosource.com/learn/southern-blotting/
21.  Freeze, HH (1993). "Preparation and analysis of glycoconjugates". Current Protocols in Molecular Biology. Chapter 17: 17.7.1–17.7.8. doi:10.1002/0471142727.mb1707s23. PMID 18265163
22. https://www.web-books.com/MoBio/Free/Ch9D.htm
23. Levéziel H. Identification of the two common alleles of the bovine κ-casein locus by the RFLP technique, using the enzyme Hind III Génétique, sélection, evolution. 1988;20:247.
24. May ST, et al. Using genomic DNA-based probe-selection to improve the sensitivity of high-density oligonucleotide arrays when applied to heterologous species Plant Methods. 2005;1:10.)
25. Nynke HD. Essential validation methods for E. coli strains created by chromosome engineering Journal of Biological Engineering. 2015;9:11.
26. https://www.mybiosource.com/learn/testing-procedures/northern-blotting/
27. https://www.researchgate.net/figure/Eastern-blotting-protocol_fig1_221913278
28. http://eagri.org/eagri50/GPBR311/lec25.pdf
29. homas; Thirumalapura, N; Crossley, EC; Ismail, N; Walker, DH; et al. (2009). "Antigenic protein modifications in Ehrlichia". Parasite Immunology. 31 (6): 296–303. doi:10.1111/j.1365-3024.2009.01099.x. PMC 2731653. PMID 19493209
30. https://images.app.goo.gl/sXVAii8dNbxw1XqZ7