Albert Science International Organization

ARTICLE DESCRIPTION: 

S. Das, Antibacterial (Bacillus Subtilis and Escherichia Coli) & Antifungal Activity (Candida Albicans) Of Ethanolic Extracts From Mentha Piperita, ASIO Journal of Microbiology, Food Science & Biotechnological Innovations (ASIO-JMFSBI), 2015, 1(1): 31-33.

ARTICLE TYPE: RESEARCH

dids/doi No.: 01.2016-97391785

dids link: http://dids.info/didslink/01.2016-79434527/

ABSTRACT:

Ethanolic extracts of whole plants of Mentha piperita was collected from various regions of Kolkata, West Bengal and this extracts was further assayed for the in vitro antibacterial activity against one Gram-positive (B. Subtilis) and one Gram-negative bacteria (Escherichia coli), using agar dilution methods. At the same time anti fungal activity of Candida albicans was determined, using both agar dilution and disc-diffusion methods. The minimum inhibition concentration (MIC) of the M. piperita, extracts was 5 mg/mL for all the microorganisms tested. The results proved that the ethanolic extract of Mentha piperita L. showed anti fungal activity against the strains Candida albicans. Mentha piperita existing considerable antimicrobial activity against the strains B. subtilis, E. coli and C. albicans.

Key words: Antimicrobial activity, whole plants, plant extracts, MIC.

1. Duarte MCT, Figueira GM, Sartoratto, A, Rehder VLG, Delarmelina C. Anti-Candida activity of Brazilian medicinal plants, Journal of Ethnopharmacology, 2005, vol. 97, p. 305-311.
2. Harbottle H, Thakur S, Zhao S, White DG. Genetics of Antimicrobial Resistance, Anim. Biotechnol., 2006, 17, 111-124.
3. Coates A, Hu Y, Bax R, Page C. The future challenges facing the developement of new antimicrobial drugs, Nat. Rev. Drug Discov., 2002, 1, 895-910.
4. Betoni JEC, Mantovani RP, Barbosa LN, Di-Stasi LC, Fernandes A. Synergism between plant extract and antimicrobial drugs used on Staphylococcus aureus diseases, Mem. Inst. Oswaldo Cruz 2006, 101, 387-390.
5. Lewis K, Ausubel FM. Prospects of plant derived antibacterials, Nat. Biotechnol., 2006, 24, 1504-1507.
6. Brantner A, Grein E. Antibacterial Activity of Plant Extracts Used Externally in Traditional Medicine, J. Ethnopharmacol., 1994, 44, 35-40.
7. Somchit MN, Reezal I, Nur IE, Mutalib AR. In vitro Antimicrobial Activity of Ethanol and Water Extracts of Cassia alata,  J. Ethnopharmacol. , 2003, 84, 1-4.
8. Indu MN, Hatha AAM, Abirosh C, Harsha U, Vivekanandan G. Antimicrobial activity of some of the south-Indian spices against serotypes of Escherichia coli, Salmonella, Listeria monocytogenes and Aeromonas hydrophila, Braz. J. Microbiol., 2006, 37: 153-158.
9. National Commitee for Clinical Laboratory Standards (NCCLS). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved Standard - Sixth Edition – NCCLS document M7-A6,Wayne, PA, USA, 2003.
10. Bonjar GHS. Inhibition of Clotrimazole-resistant Candida albicans by plants used in Iranian folkloric. Fitoterapia, 2004,75(1):74-6.
11. Tampieri MP, Galuppi R, Macchioni F, Carelle MS, Falcioni L, Cioni PL. The inhibition of Candida albicans by selected essential oils and their major components. Mycopathologi,. 2005, 159(3):339-45.